Test Guidelines
Visit OECD’s Guidelines for the Testing of Chemicals for a complete list of guidelines.
Alternative non-animal test guidelines
Test No. 428: Skin Absorption: In Vitro Methods
Test No. 430: In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test Method (TER)
Test No. 431: In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method
Test No. 432: In Vitro 3T3 NRU Phototoxicity Test
Test No. 435: In Vitro Membrane Barrier Test Method for Skin Corrosion
Test No. 439: In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method
Test No. 442C: In Chemico Skin Sensitization
Test No. 442D: In Vitro Skin Sensitisation
- ARE-Nrf2 luciferase KeratinoSens™ test method
- ARE-Nrf2 luciferase LuSens test method
- EpiSensA
- Epi2SensA
- ARE‑Nrf2 luciferase α‑Sens test method
Test No. 444A: In Vitro Immunotoxicity, the IL-2 Luc Assay
- IL-2 Luc Assay
- IL-2 Luc Leukocyte Toxicity Test (LTT) Assay
Test No. 445A: Determination of Cytochrome P450 (CYP) Induction using Differentiated Human Hepatic Cells
Test No. 446A: ToxTracker gene reporter assay for the identification of genotoxic hazard and genotoxic/non‑genotoxic mechanism of action
Test No. 454A: Human Glucocorticoid Receptor Transactivation Assay
Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists; accompanying software is also available
Test No. 467: Defined Approaches for Serious Eye Damage and Eye Irritation
Test No. 473: In Vitro Mammalian Chromosomal Aberration Test
Test No. 476: In Vitro Mammalian Cell Gene Mutation Tests Using the HPRT and XPRT Genes
Test No. 479: Genetic Toxicology: In Vitro Sister Chromatid Exchange Assay in Mammalian Cells
Test No. 482: Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells In Vitro
Test No. 487: In Vitro Mammalian Cell Micronucleus Test
Test No. 490: In Vitro Mammalian Cell Gene Mutation Test Using the Thymidine Kinase Gene
Test No. 492B: Reconstructed Human Cornea-Like Epithelium (RHCE) Test Method for Eye Hazard Identification
Test No. 493: Performance-Based Test Guideline for Human Recombinant Estrogen Receptor (hrER) In Vitro Assays to Detect Chemicals with ER Binding Affinity
Guideline No. 497: Defined Approaches on Skin Sensitisation
Test No. 498: In vitro Phototoxicity ‑ Reconstructed Human Epidermis Phototoxicity test method